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绵羊痘的病原检测结论与参考文献

来源:学术堂 作者:姚老师
发布于:2015-03-10 共7404字
  本篇论文快速导航:
第1部分 一例绵羊痘的分离检测研究
第2部分 绵羊痘相关研究文献综述
第3部分 一起地方绵羊痘的实验室诊断
第4部分 绵羊痘病毒辽宁锦州分离株的分离鉴定
第5部分 绵羊痘的病原检测结论与参考文献

展开更多

  结论

  1. 应用病理形态学观察、电镜负染、PCR 检测等方法从病理学和病原学角度对临床上一起疑似绵羊痘感染疫情进行诊断,确诊为 SPPV 感染所致。
  2. 利用原代胎羊肾细胞从感染病羊肺部痘疹组织中成功分离获得 1 株绵羊痘病毒,将其命名为 SPPV/LN/12/JZ 分离株。
  3. P32 基因的核苷酸序列同源性及遗传进化分析结果表明,SPPV/LN/12/JZ 分离株与宁夏绵羊 SPPV 分离株(JN596274)同源性最高,且处于同一进化小分支。

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